CALL FOR PAPERS Cell Signaling: Proteins, Pathways and Mechanisms A practical method for monitoring FRET-based biosensors in living animals using two-photon microscopy
نویسندگان
چکیده
Wen Tao, Michael Rubart, Jennifer Ryan, Xiao Xiao, Chunping Qiao, Takashi Hato, Michael W. Davidson, Kenneth W. Dunn, and Richard N. Day Department of Medicine, Division of Nephrology, Indiana University Medical Center, Indianapolis, Indiana; Riley Heart Research Center, Wells Center for Pediatric Research, and Krannert Institute of Cardiology, Indiana University School of Medicine, Indianapolis, Indiana; National High Magnetic Field Laboratory and Department of Biological Science, The Florida State University, Tallahassee, Florida; Division of Molecular Pharmaceutics, Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina; and Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, Indiana
منابع مشابه
A practical method for monitoring FRET-based biosensors in living animals using two-photon microscopy.
The commercial availability of multiphoton microscope systems has nurtured the growth of intravital microscopy as a powerful technique for evaluating cell biology in the relevant context of living animals. In parallel, new fluorescent protein (FP) biosensors have become available that enable studies of the function of a wide range of proteins in living cells. Biosensor probes that exploit Först...
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A Practical Method for Monitoring FRET-based Biosensors in Living Animals 1 Using Two-photon Microscopy 2 3 Wen Tao, Michael Rubart, Jennifer Ryan, Xiao Xiao, Chunping Qiao, Takashi Hato, Michael W. 4 Davidson, Kenneth W. Dunn, Richard N. Day* 5 6 Department of Medicine; Division of Nephrology; Indiana University Medical Center; Indianapolis, IN 7 USA 8 Riley Heart Research Center, Wells Center...
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UNDERSTANDING MECHANISMS of physiologic and pathophysiologic processes requires their investigation at the cellular and molecular level within the genuine environment—the living organism. In the past two decades, two-photon laser-scanning microscopy (TPLSM) has become, because of the increased tissue penetration and lesser scattering of the longer wavelengths used in two-photon excitation (3), ...
متن کاملMonitoring Biosensor Activity in Living Cells with Fluorescence Lifetime Imaging Microscopy
Live-cell microscopy is now routinely used to monitor the activities of the genetically encoded biosensor proteins that are designed to directly measure specific cell signaling events inside cells, tissues, or organisms. Most fluorescent biosensor proteins rely on Förster resonance energy transfer (FRET) to report conformational changes in the protein that occur in response to signaling events,...
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Among biosensors, genetically-encoded FRET biosensors raised hope to dissect signaling pathways by monitoring enzymatic activities or second messengers levels in living cells and even in living and developing organisms. FRET (Froster resonance Energy Transfer) is a radiationless energy transfer from donor to acceptor fluorophore. For most genetically encoded donor/acceptor pair, this transfer c...
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تاریخ انتشار 2015